Collagen I Antibody / Extracellular Matrix Protein (R32379)

Collagen I Antibody Human Placenta IF. Immunofluorescent staining of FFPE human placental tissue with Collagen I antibody revealed strong extracellular and stromal staining consistent with the distribution of type I collagen within connective tissue compartments. Collagen I, composed primarily of COL1A1 and COL1A2 chains, is the most abundant fibrillar collagen in the extracellular matrix and provides structural support, tensile strength, and tissue integrity. Red fluorescence indicates Collagen I expression, while nuclei are counterstained with DAPI (blue). The prominent extracellular staining pattern observed in placental connective tissues is consistent with the essential role of collagen I in matrix organization, tissue architecture, and cell-matrix interactions. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to immunostaining. These results support the utility of Collagen I Antibody for studies of extracellular matrix biology, tissue remodeling, fibrosis, and connective tissue development.

Collagen I Antibody Mouse Lung IF. Immunofluorescent staining of FFPE mouse lung tissue with Collagen I antibody revealed strong extracellular staining associated with connective tissue structures and alveolar septal regions, consistent with the distribution of type I collagen within the pulmonary extracellular matrix. Collagen I, composed primarily of COL1A1 and COL1A2 chains, is a major fibrillar collagen that provides structural support and mechanical stability to connective tissues. Red fluorescence indicates Collagen I expression, while nuclei are counterstained with DAPI (blue). The prominent staining pattern along stromal and matrix-rich regions is consistent with the role of collagen I in maintaining lung architecture and regulating tissue remodeling. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to immunostaining. These results support the utility of Collagen I Antibody for studies of extracellular matrix biology, pulmonary fibrosis, tissue remodeling, and connective tissue development.

Collagen I Antibody Rat Lung IF. Immunofluorescent staining of FFPE rat lung tissue with Collagen I antibody revealed strong extracellular staining associated with peribronchial, perivascular, and interstitial connective tissue structures, consistent with the distribution of type I collagen within the pulmonary extracellular matrix. Collagen I, composed primarily of COL1A1 and COL1A2 chains, is the most abundant fibrillar collagen in vertebrate tissues and provides structural support, tensile strength, and matrix integrity. Red fluorescence indicates Collagen I expression, while nuclei are counterstained with DAPI (blue). The prominent staining observed within matrix-rich regions of the lung is consistent with the role of collagen I in maintaining tissue architecture and regulating extracellular matrix remodeling. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to immunostaining. These results support the utility of Collagen I Antibody for studies of extracellular matrix biology, pulmonary fibrosis, tissue remodeling, and connective tissue development.

IHC staining of FFPE human placental tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder urothelial carcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human renal cell carcinoma tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse lung tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat lung tissue with Collagen I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human placenta, 2) rat skin, 3) rat lung, 4) mouse skin and 5) mouse NIH 3T3 cell lysate with Collagen I antibody. Expected molecular weight: 140-210 kDa (precusor), 70-90 kDa (mature).