Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody (R31258)

Collagen 1 Antibody for IF Placenta. Immunofluorescence analysis of Collagen I / COL1A1 expression in FFPE human placental tissue using Collagen 1 antibody. This Collagen 1 antibody produces strong extracellular red fluorescence highlighting fibrillar collagen deposition within stromal and vascular regions, outlining connective tissue architecture, while DAPI (blue) marks nuclei. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 10-20 min followed by cooling prior to immunofluorescence staining.

Collagen 1 Antibody for IF Mouse Lung. Immunofluorescence analysis of Collagen I / COL1A1 expression in FFPE mouse lung tissue using Collagen 1 antibody. This Collagen 1 antibody shows strong extracellular red fluorescence outlining peribronchial and perivascular connective tissue structures, consistent with fibrillar collagen deposition, while DAPI (blue) marks nuclei. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 10-20 min followed by cooling prior to immunofluorescence staining.

Collagen 1 Antibody for IF Rat Lung. Immunofluorescence analysis of Collagen I / COL1A1 expression in FFPE rat lung tissue using Collagen 1 antibody. This Collagen 1 antibody reveals prominent extracellular red fluorescence distributed along interstitial and peribronchial regions, consistent with fibrillar collagen networks within lung connective tissue, while DAPI (blue) marks nuclei. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 10-20 min followed by cooling prior to immunofluorescence staining.

Collagen 1 Antibody for IF Breast Tissue. Immunofluorescence analysis of Collagen I / COL1A1 expression in FFPE human breast tissue using Collagen 1 antibody. This Collagen 1 antibody highlights extracellular red fluorescence outlining stromal collagen fibers surrounding epithelial structures and ducts, consistent with fibrillar matrix organization, while DAPI (blue) marks nuclei. Antigen retrieval was performed by boiling sections in pH 6 citrate buffer for 10-20 min followed by cooling prior to immunofluorescence staining.

Collagen 1 Antibody for IF Tonsil. Immunofluorescence analysis of Collagen I / COL1A1 expression in FFPE human tonsil tissue using Collagen 1 antibody. This Collagen 1 antibody shows extracellular red fluorescence distributed within stromal and interfollicular connective tissue regions, outlining structural support networks surrounding lymphoid aggregates, while DAPI (blue) marks nuclei. Antigen retrieval was performed by boiling sections in pH 6 citrate buffer for 10-20 min followed by cooling prior to immunofluorescence staining.

Collagen 1 Antibody Liver Cancer IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human liver cancer tissue using Collagen 1 antibody. Prominent HRP-DAB brown staining highlights dense extracellular collagen deposition within tumor-associated stroma, outlining fibrous septa and stromal compartments surrounding tumor epithelial cells, while adjacent tumor cells show minimal intracellular signal; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody Renal Cell Carcinoma IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human renal cell carcinoma tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen fibers outlining tumor-associated stroma and vascular structures, forming a delicate network surrounding nests of tumor cells, while tumor cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody Bladder Urothelial Carcinoma IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human bladder urothelial carcinoma tissue using Collagen 1 antibody. HRP-DAB brown staining highlights dense extracellular collagen fibers within tumor-associated stroma, forming interlacing fibrous networks surrounding tumor cell clusters, while malignant urothelial cells show minimal intracellular signal; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody Placenta IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human placental tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen deposition outlining chorionic villi and stromal compartments, forming continuous fibrous structures around placental tissue architecture, while trophoblast cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody Esophageal Squamous Carcinoma IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human esophageal squamous carcinoma tissue using Collagen 1 antibody. HRP-DAB brown staining highlights dense extracellular collagen fibers within tumor-associated stroma, forming fibrous bands surrounding nests of squamous tumor cells, while malignant epithelial cells show minimal intracellular signal; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody Mouse Lung IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE mouse lung tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen fibers outlining bronchial and perivascular structures, forming a supportive connective tissue framework within the lung parenchyma, while surrounding alveolar epithelial cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody Rat Lung IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE rat lung tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen fibers concentrated around bronchial and perivascular regions, forming organized connective tissue structures within the lung parenchyma, while surrounding alveolar epithelial cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody NIH3T3 ICC. Immunocytochemistry analysis of Collagen I / COL1A1 expression in NIH3T3 cells using Collagen 1 antibody. HRP-DAB brown staining is observed within the extracellular matrix and pericellular regions, consistent with collagen deposition and secretion by fibroblast cells, while nuclei are counterstained blue. Antigen retrieval was performed by boiling samples in pH 6 citrate buffer for 10-20 min followed by cooling prior to staining.

Collagen 1 Antibody Tissue WB. Western blot analysis of Collagen I / COL1A1 expression using Collagen 1 antibody. Lane 1: human placenta lysate, Lane 2: rat skin lysate, Lane 3: rat lung lysate, Lane 4: mouse skin lysate, Lane 5: mouse NIH3T3 cell lysate. Bands are detected in the range of approximately 140-210 kDa, consistent with the predicted molecular weight of procollagen I, with additional bands observed around 70-90 kDa corresponding to processed collagen forms generated during maturation.