Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody (R31258)

Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody Human Endometrial Cancer IF. Immunofluorescent staining of FFPE human endometrial cancer tissue using Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody. Collagen I is detected predominantly within the extracellular matrix surrounding tumor glands, consistent with its role as a major structural component of the stromal microenvironment. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by Fluoro550-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI and fluorescence was visualized using appropriate filter sets.

Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody Human Intestinal Cancer IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody. Collagen I is detected predominantly within the desmoplastic stromal extracellular matrix surrounding malignant glands, consistent with its role in maintaining tumor architecture and supporting the tumor microenvironment. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by Cy3-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI and fluorescence was visualized using appropriate filter sets.

Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody Rat Lung IF. Immunofluorescent staining of FFPE rat lung tissue using Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody. Collagen I is detected predominantly within the extracellular matrix of the pulmonary interstitium, consistent with its role in maintaining lung structural integrity and supporting connective tissue architecture. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by a Cy3-conjugated goat anti-rabbit IgG secondary antibody. Nuclei were counterstained with DAPI and fluorescence was visualized using appropriate filter sets.

Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody NIH/3T3 Cell IF. Immunofluorescent staining of NIH/3T3 cells using Collagen 1 Antibody for IF / COL1A1 Immunofluorescence Antibody. Collagen I is detected predominantly within the cytoplasm and pericellular extracellular matrix, consistent with its synthesis and secretion by fibroblast-like cells. Enzymatic antigen retrieval was performed for 15 minutes prior to staining. Cells were blocked with 10% goat serum and incubated with the primary antibody (5 ug/ml) overnight at 4°C, followed by a Fluoro488-conjugated goat anti-rabbit IgG secondary antibody. Nuclei were counterstained with DAPI and fluorescence was visualized using appropriate filter sets.

Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody Human Esophageal Squamous Cell Carcinoma IHC. Immunohistochemical staining of FFPE human esophageal squamous cell carcinoma tissue using Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody. Collagen I is detected predominantly within the stromal extracellular matrix surrounding tumor nests, consistent with its role in maintaining tissue architecture and supporting the tumor microenvironment. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

Collagen 1 Antibody Renal Cell Carcinoma IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human renal cell carcinoma tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen fibers outlining tumor-associated stroma and vascular structures, forming a delicate network surrounding nests of tumor cells, while tumor cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody Human Bladder Cancer IHC. Immunohistochemical staining of FFPE human bladder cancer tissue using Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody. Collagen I is detected predominantly within the stromal extracellular matrix surrounding tumor nests, consistent with its role in providing structural support and shaping the tumor microenvironment. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

Collagen 1 Antibody Placenta IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE human placental tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen deposition outlining chorionic villi and stromal compartments, forming continuous fibrous structures around placental tissue architecture, while trophoblast cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody Human Rectal Adenocarcinoma IHC. Immunohistochemical staining of FFPE human rectal adenocarcinoma tissue using Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody. Collagen I is detected predominantly within the stromal extracellular matrix surrounding malignant glands, consistent with its role in maintaining tissue architecture and contributing to the desmoplastic tumor microenvironment. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

Collagen 1 Antibody Mouse Lung IHC. Immunohistochemistry analysis of Collagen I / COL1A1 expression in FFPE mouse lung tissue using Collagen 1 antibody. HRP-DAB brown staining highlights extracellular collagen fibers outlining bronchial and perivascular structures, forming a supportive connective tissue framework within the lung parenchyma, while surrounding alveolar epithelial cells show minimal intracellular staining; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 min followed by cooling prior to staining.

Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody Multi-Species WB. Western blot analysis of 1) human placenta tissue, 2) rat skin tissue, 3) mouse skin tissue, and 4) mouse NIH/3T3 cell lysates using Collagen 1 Antibody / COL1A1 Extracellular Matrix Antibody. Specific immunoreactive bands are detected at approximately 130, 220, and 250 kDa, consistent with the expected migration of Collagen I alpha 1 and higher molecular weight collagen species. The predicted molecular weight of the COL1A1 alpha chain is approximately 138 kDa.