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Home >> Antibodies >> COL4A1 Antibody for IHC / Collagen type IV alpha 1 chain

COL4A1 Antibody for IHC / Collagen type IV alpha 1 chain [clone MSVA-704R] (V6065)

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  Catalog No Formulation Size Price (USD)  
Image V6065-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
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V6065-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry analysis using COL4A1 antibody for IHC with human tissue microarrays. Formalin-fixed, paraffin-embedded normal and cancer human tissues were stained using recombinant rabbit monoclonal COL4A1 antibody (clone MSVA-704R). Strong HRP-DAB brown chromogenic signal highlights basement membrane structures surrounding epithelial glands, vascular endothelium, and stromal interfaces, consistent with extracellular localization of Collagen type IV alpha 1 chain. Across normal tissues, linear basement membrane staining is observed in kidney, lung, gastrointestinal tract, and skin, while in selected carcinomas, staining outlines preserved or disrupted basement membrane architecture. The overall staining pattern aligns with published expression data in the Human Protein Atlas.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-704R
UniProt P02462 , P08572 , P29400 , P53420
Localization Basement membrane, Extracellular matrix, Extracellular space, Secreted
Applications Immunohistochemistry : 1:100-1:200
Limitations This COL4A1/Collagen type IV alpha 1 chain antibody is available for research use only.
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Description

COL4A1 Antibody for IHC detects Collagen type IV alpha 1 chain, a major structural component of basement membranes encoded by the COL4A1 gene. This recombinant monoclonal antibody is designed for immunohistochemistry applications and enables evaluation of basement membrane integrity and extracellular matrix architecture in formalin-fixed tissue sections.

COL4A1 antibody, also referred to as Collagen IV antibody and Type IV collagen antibody in the literature, recognizes the alpha 1 chain that participates in formation of the Collagen IV heterotrimeric network. Collagen type IV alpha 1 associates with alpha 2 chains to form the predominant alpha 1 alpha 1 alpha 2 protomer found in most basement membranes. Unlike fibrillar collagens, Collagen IV forms a sheet-like scaffold that provides mechanical stability and structural compartmentalization between epithelial, endothelial, and stromal compartments. These networks interact with laminins, nidogens, and heparan sulfate proteoglycans to regulate cell adhesion, differentiation, migration, and barrier function.

COL4A1 is widely expressed in vascular basement membranes, kidney glomeruli, lung alveoli, skeletal muscle endomysium, and epithelial basement membrane zones across many organs. In immunohistochemical analyses, Collagen type IV alpha 1 typically demonstrates linear brown staining outlining blood vessels, glandular structures, and epithelial-stromal interfaces. This characteristic basement membrane pattern supports studies of angiogenesis, tumor invasion, and extracellular matrix remodeling in both developmental and disease contexts.

Pathogenic variants in COL4A1 have been linked to small vessel disease, cerebral hemorrhage susceptibility, ocular defects, and renal abnormalities. In cancer research, discontinuity or loss of Collagen IV staining may indicate basement membrane disruption and invasive potential. A COL4A1 Antibody for IHC can therefore assist in investigations of vascular pathology, basement membrane biology, and tissue microenvironment changes. This antibody targets Collagen type IV alpha 1 in research applications and is available from NSJ Bioreagents.

Application Notes

1. Optimal dilution of the COL4A1 antibody for IHC should be determined by the researcher.

2. This COL4A1/Collagen type IV alpha 1 chain antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

Purified human placental extract was used as the immunogen for the COL4A1 antibody for IHC.

Storage

COL4A1/Collagen type IV alpha 1 chain antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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