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Home >> Antibodies >> COL4A1 Antibody / Collagen type IV alpha 1 chain

COL4A1 Antibody / Collagen type IV alpha 1 chain [clone MSVA-704R] (V6065)

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  Catalog No Formulation Size Price (USD)  
Image V6065-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6065-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Collagen alpha-1(IV) chain Rabbit Recombinant Monoclonal Antibody (MSVA-704R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-704R
UniProt P02462 , P08572 , P29400 , P53420
Localization Basement membrane, Extracellular matrix, Extracellular space, Secreted
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This COL4A1/Collagen type IV alpha 1 chain antibody is available for research use only.
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Application Notes

1. Optimal dilution of the COL4A1/Collagen type IV alpha 1 chain antibody should be determined by the researcher.

2. This COL4A1/Collagen type IV alpha 1 chain antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

Purified human placental extract was used as the immuongen for the COL4A1/Collagen type IV alpha 1 chain antibody.

Storage

COL4A1/Collagen type IV alpha 1 chain antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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