Cofilin 2 Antibody / CFL2 [clone 8C13] (RQ5498)

Immunofluorescent staining of FFPE human U-2 OS cells with Cofilin 2 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE human skeletal muscle with Cofilin 2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE mouse skeletal muscle with Cofilin 2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

IHC staining of FFPE rat skeletal muscle with Cofilin 2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa, 2) U-2 OS, 3) HepG2, 4) T-47D, 5) Raji, 6) placenta and 7) A549 lysate with Cofilin 2 antibody. Predicted molecular weight ~19 kDa.

Western blot testing of 1) rat heart, 2) rat liver, 3) rat kidney, 4) rat brain, 5) mouse heart, 6) mouse liver, 7) mouse kidney, 8) mouse brain and 9) mouse NIH3T3 lysate with Cofilin 2 antibody. Predicted molecular weight ~19 kDa.

Flow cytometry testing of human A549 cells with Cofilin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cofilin 2 antibody.

Flow cytometry testing of human SiHa cells with Cofilin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cofilin 2 antibody.