- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
COBRA1 Antibody / Cofactor of BRCA1 / NELFB (RQ4476)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ4476 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | Q8WX92 |
| Localization | Nucleus |
| Applications | Western Blot : 0.5-1ug/ml Direct ELISA : 0.1-0.5ug/ml (human recombinant protein) |
| Limitations | This COBRA1 antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
COBRA1 antibody detects Cofactor of BRCA1, a nuclear protein that functions as a transcriptional coregulator and chromatin-associated factor. The UniProt recommended name is Cofactor of BRCA1 (COBRA1). This protein plays an important role in transcriptional control, DNA damage response, and gene silencing by acting as a subunit of the negative elongation factor (NELF) complex, which regulates RNA polymerase II pausing during transcription elongation.
Functionally, COBRA1 antibody identifies a 603-amino-acid nuclear protein that interacts with BRCA1, linking it to DNA repair and cell cycle regulation. Within the NELF complex, COBRA1 (also referred to as NELF-B) associates with NELF-A, NELF-C/D, and NELF-E to repress transcription elongation at specific promoter regions. This pausing mechanism allows cells to fine-tune gene expression in response to developmental cues and environmental stress. COBRA1 is also involved in chromatin remodeling and epigenetic regulation, where it helps maintain proper nucleosome positioning and transcriptional fidelity.
The COBRA1 gene (officially known as NELFB) is located on chromosome 9q34.3 and is expressed in a variety of tissues including liver, testis, and lung, with high nuclear localization. It was originally identified as a BRCA1-interacting protein, and subsequent studies have shown that its association with BRCA1 contributes to tumor suppression by stabilizing gene expression networks that preserve genomic integrity. In addition, COBRA1 is implicated in the control of estrogen receptor signaling and developmental transcriptional programs.
Pathologically, altered expression of COBRA1 has been linked to cancer development and progression. Decreased COBRA1 expression has been observed in breast and ovarian cancers, where loss of its repressive function can promote uncontrolled transcription and proliferation. Conversely, overexpression may disrupt normal transcriptional balance. Its role in transcriptional pausing also connects it to viral gene regulation and cellular stress responses. Research using COBRA1 antibody supports studies in transcriptional regulation, BRCA1-related tumor suppression, and chromatin biology.
COBRA1 antibody is validated for use in relevant research applications to detect NELF complex components and investigate transcriptional control mechanisms. NSJ Bioreagents provides COBRA1 antibody reagents optimized for studies in gene regulation, DNA repair, and cancer biology.
Optimal dilution of the COBRA1 antibody should be determined by the researcher.
Human COBRA1 recombinant protein (amino acids M1-E252) was used as the immunogen for the COBRA1 antibody.
After reconstitution, the COBRA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
