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Home >> Antibodies >> CNP Antibody / CNPase

CNP Antibody / CNPase (RQ7511)

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Image RQ7511 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE rat brain tissue with CNP antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE rat brain tissue with CNP antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE mouse brain tissue with CNP antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with CNP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with CNP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human U-251, 3) human HepG2, 4) human MOLT4, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse Neuro-2a cell lysate with CNP antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human U937 cells with CNP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CNP antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P09543
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This CNP antibody is available for research use only.
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Description

2',3'-Cyclic-nucleotide 3'-phosphodiesterase, also known as CNPase, is an enzyme that in humans is encoded by the CNP gene. And this gene is mapped to 17q21.2. CNPase is named for its ability to catalyze the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. CNPase is thought to play a critical role in the events leading up to myelination. Additionally, CNPase has been demonstrated to inhibit the replication of HIV-1 and other primate lentiviruses by binding the retroviral Gag protein and inhibiting the genesis of nascent viral particles.

Application Notes

Optimal dilution of the CNP antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-I421) was used as the immunogen for the CNP antibody.

Storage

After reconstitution, the CNP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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