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Home >> Antibodies >> CMBL Antibody

CMBL Antibody (RQ6089)

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  Catalog No Formulation Size Price (USD)  
Image RQ6089 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human lung cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adnexal serous adenocarcinoma tissue with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) PC-3, 3) HepG2, 4) HEK293, 5) A549, 6) U-87 MG, 7) rat liver, 8) rat kidney and 9) mouse kidney lysate with CMBL antibody. Predicted molecular weight ~28 kDa.
Flow cytometry testing of human U-2 OS cells with CMBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CMBL antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q96DG6
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This CMBL antibody is available for research use only.
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  • Applications : WB, FACS
    Reactivity : Mouse, Rat

Description

CMBL is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom.

Application Notes

Optimal dilution of the CMBL antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids M1-M246) was used as the immunogen for the CMBL antibody.

Storage

After reconstitution, the CMBL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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