CLU Antibody / Clusterin [clone CLU/4737] (V5859)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human adrenal gland tissue using CLU/Clusterin antibody (clone CLU/4737). Predominantly cytoplasmic staining is observed in adrenal cortical cells, with variable intensity across cell populations and minimal background staining in surrounding stromal areas. Nuclei are counterstained in blue. Heat-induced antigen retrieval was performed by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC, followed by cooling at room temperature for 20 minutes.

Analysis of Protein Array containing more than 19,000 full-length human proteins using CLU/Clusterin antibody (clone CLU/4737).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free CLU/Clusterin antibody (clone CLU/4737) as confirmation of integrity and purity.