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Home >> Antibodies >> CLPX Antibody

CLPX Antibody [clone 11C11] (RQ5618)

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  Catalog No Formulation Size Price (USD)  
Image RQ5618 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IF/ICC staining of FFPE human MCF7 cells with CLPX antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IF/ICC staining of FFPE human MCF7 cells with CLPX antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with CLPX antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CLPX antibody.
Western blot testing of human 1) Raji, 2) HeLa, 3) HepG2 and 4) Caco-2 lysate with CLPX antibody. Predicted molecular weight ~69 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 11C11
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O76031
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This CLPX antibody is available for research use only.
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Description

ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.

Application Notes

Optimal dilution of the CLPX antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids A337-E574) was used as the immunogen for the CLPX antibody.

Storage

After reconstitution, the CLPX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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