CLPX Antibody / Caseinolytic Protease X Antibody (RQ4230)

CLPX Antibody IHC in Human Liver. Immunohistochemistry of FFPE human liver tissue was performed with CLPX Antibody at 1:200 dilution and visualized with DAB. Strong brown cytoplasmic staining is visible throughout the hepatocyte population, with heterogeneous intensity among individual cells and comparatively little nuclear signal. The widespread cytoplasmic staining supports tissue detection using a Caseinolytic Protease X Antibody.

CLPX Antibody IHC in Human Brain. Immunohistochemistry of FFPE human brain tissue was performed with CLPX Antibody at 1:200 dilution and visualized with DAB. Brown staining is distributed throughout the neuropil and within neuronal cell bodies, with heterogeneous cytoplasmic labeling among individual cells. The staining demonstrates detection of caseinolytic protease X in human brain tissue.

CLPX Antibody Immunofluorescence in U2OS Cells. Human U2OS cells were stained with CLPX Antibody at 1:100 dilution (green) and counterstained with DAPI (blue). CLPX fluorescence forms a prominent punctate and reticular cytoplasmic pattern surrounding the nuclei, with minimal signal within the DAPI-positive nuclear compartment. The distribution is consistent with the expected mitochondrial localization of CLPX.

CLPX Antibody Western Blot in Human, Rat and Mouse Samples. Western blot testing of human HepG2 (lane 1), A549 (lane 2), HeLa (lane 3), and HT1080 (lane 4) cell lysates, rat liver (lane 5) and heart (lane 6), and mouse liver (lane 7) and heart (lane 8) tissue lysates was performed with CLPX Antibody at 1:1000 dilution. A predominant band is detected at approximately 60-65 kDa across all eight samples, somewhat below the stated expected molecular weight of ~69 kDa. Signal intensity varies among samples, with particularly strong detection in the human cell lysates and mouse liver. The broad cross-species detection supports recognition using a Caseinolytic Protease X Antibody.