Claudin 7 Antibody / Tight Junction Protein Antibody (RQ6250)

Claudin 7 Antibody Rectal Cancer Tissue IF. Immunofluorescence analysis of FFPE human rectal cancer stained with Claudin 7 Antibody (green) demonstrates prominent membranous localization in tumor epithelial cells consistent with tight junction distribution, while nuclei are counterstained with DAPI (blue); heat-induced epitope retrieval was performed in pH 8 EDTA buffer.

Claudin 7 Antibody MCF7 Cells IF. Immunofluorescence analysis of human MCF7 cells stained with Claudin 7 Antibody (green) demonstrates distinct membranous localization at cell-cell junctions consistent with tight junction organization, while nuclei are counterstained with DAPI (blue); heat-induced epitope retrieval was performed in pH 8 EDTA buffer.

Claudin 7 Antibody Renal Clear Cell Carcinoma Tissue IHC. Immunohistochemistry analysis of FFPE human renal clear cell carcinoma stained with Claudin 7 Antibody demonstrates HRP-DAB brown membranous staining outlining tumor epithelial cells, consistent with tight junction-associated localization, with weaker cytoplasmic signal and minimal background in surrounding stromal elements; antigen retrieval was performed in pH 8 EDTA buffer.

Claudin 7 Antibody Rectal Cancer Tissue IHC. Immunohistochemistry analysis of FFPE human rectal cancer stained with Claudin 7 Antibody demonstrates strong HRP-DAB brown membranous staining in tumor epithelial cells consistent with tight junction distribution, with mild cytoplasmic signal and low background in adjacent stromal tissue; antigen retrieval was performed in pH 8 EDTA buffer.

Claudin 7 Antibody Gallbladder Adenocarcinoma Tissue IHC. Immunohistochemistry analysis of FFPE human gallbladder adenocarcinoma stained with Claudin 7 Antibody demonstrates HRP-DAB brown membranous staining in tumor epithelial cells consistent with tight junction localization, with focal cytoplasmic signal and low background in surrounding stromal tissue; antigen retrieval was performed in pH 8 EDTA buffer.

Claudin 7 Antibody Caco-2 WB. Western blot analysis of human Caco-2 cell lysate using Claudin 7 Antibody detects a band at approximately 20-25 kDa, consistent with the predicted molecular weight of Claudin 7, along with an additional band near ~35-40 kDa, which may reflect dimerization, post-translational modification, or SDS-resistant oligomeric forms commonly observed for tight junction proteins.

Claudin 7 Antibody Caco-2 Cell FACS. Flow cytometry testing of human Caco-2 cells with Claudin 7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Claudin 7 antibody.