Claudin 3 Antibody | CLDN3 | RQ6414 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ6414	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Claudin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with Claudin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colonic adenocarcinoma tissue with Claudin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human renal clear cell carcinoma tissue with Claudin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human gallbladder adenocarcinoma tissue with Claudin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast invasive ductal cancer tissue with Claudin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human MCF7 cells with Claudin 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) MCF7 and 2) Caco-2 cell lysate with Claudin 3 antibody. Predicted molecular weight ~23 kDa.

Flow cytometry testing of human MCF7 cells with Claudin 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Claudin 3 antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	O15551
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence (FFPE) : 5ug/ml Flow cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This Claudin 3 antibody is available for research use only.
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Description

Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.

Application Notes

Optimal dilution of the CLDN3 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A101-V220) was used as the immunogen for the Claudin 3 antibody.

Storage

After reconstitution, the Claudin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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Claudin 3 Antibody / CLDN3 (RQ6414)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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