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Home >> Antibodies >> CLASP1 Antibody / CLIP-associating protein 1

CLASP1 Antibody / CLIP-associating protein 1 (RQ7358)

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  Catalog No Formulation Size Price (USD)  
Image RQ7358 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with CLASP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human prostate cancer tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE squamous cell carcinoma of human cervix tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with CLASP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human HeLa, 3) human HEL, 4) human K562, 5) rat brain and 6) rat C6 cell lysate with CLASP1 antibody. Expected molecular weight: 162-169 kDa (multiple isoforms).
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q7Z460
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This CLASP1 antibody is available for research use only.
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Description

Cytoplasmic linker associated protein 1, also known as CLIP-associating protein 1 and CLASP1, is a protein which in humans is encoded by the CLASP1 gene. CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle.

Application Notes

Optimal dilution of the CLASP1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-D243) was used as the immunogen for the CLASP1 antibody.

Storage

After reconstitution, the CLASP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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