- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CKB Antibody for WB Multi-Species Clone CPTC-CKB-2 / Creatine Phosphokinase-BB [clone CPTC-CKB-2] (V7682)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V7682-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V7682-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
|
V7682SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | CPTC-CKB-2 |
| Purity | Protein G affinity chromatography |
| UniProt | P12277 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 2-4ug/ml |
| Limitations | This CKB Antibody for WB Multi-Species Clone CPTC-CKB-2 is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Creatine kinase B (CKB) is a cytosolic enzyme that plays a fundamental role in cellular energy buffering by catalyzing the reversible conversion of phosphocreatine and ADP into ATP and creatine. This reaction is essential in tissues with high and fluctuating energy demands, including brain, epithelial, and proliferative cell systems where rapid ATP regeneration is required. CKB Antibody for WB is widely used to detect this enzyme in protein lysates, supporting comparative analysis of energy metabolism across multiple species and experimental models. This Creatine Kinase B Western Blot Antibody is part of a broader Creatine Kinase B antibody for WB panel offered by NSJ Bioreagents.
CKB belongs to the creatine kinase family of phosphotransferases, which includes cytosolic and mitochondrial isoforms that together maintain intracellular ATP homeostasis. The B-type subunit forms homodimers (CK-BB) or heterodimers with muscle-type CKM, enabling tissue-specific metabolic regulation. CKB antibody, also known as Creatine kinase B antibody or Brain creatine kinase antibody in the literature, supports selective detection of the B isoform, which is particularly important in studies distinguishing neuronal and non-muscle metabolic pathways from muscle-derived CK activity.
Functionally, CKB operates within the phosphocreatine shuttle, a rapid-response energy transfer system that distributes high-energy phosphate groups from mitochondria to sites of ATP consumption. This process supports essential cellular functions including ion transport, cytoskeletal dynamics, and signal transduction. In western blot workflows, CKB serves as a robust metabolic marker whose expression can reflect changes in cellular energy state, proliferation status, or stress response. CKB Antibody for WB is therefore valuable for monitoring metabolic adaptations across diverse biological systems.
Subcellularly, CKB is predominantly localized in the cytoplasm but can associate with membrane structures and protein complexes that require localized ATP regeneration. This distribution contributes to consistent extraction into soluble protein fractions and reliable detection in western blot analysis. The protein’s relatively stable expression and clear electrophoretic behavior support reproducible band identification across different sample types and species, making it particularly suitable for cross-species lysate comparison studies.
This CKB Antibody for WB is supported by western blot data demonstrating detection of endogenous CKB protein across multiple species, enabling direct comparison of expression profiles in human and common model organisms. In addition, protein microarray specificity validation confirms highly selective binding to CKB among thousands of human proteins, providing strong confidence in specificity and minimizing off-target detection. Together, these validation approaches make this antibody a dependable reagent for multi-species western blot analysis in studies of energy metabolism, neuronal biology, and cellular physiology.
This Creatine Kinase B Antibody part of a broader Creatine Kinase B antibody panel offered by NSJ Bioreagents.
Optimal dilution of the CKB Antibody for WB Multi-Species Clone CPTC-CKB-2 should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the CKB antibody.
Store the CKB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Creatine kinase B antibody, Brain creatine kinase antibody, CK-BB antibody, Creatine kinase B chain antibody, Cytosolic creatine kinase B antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
