- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CKB Antibody / CKBB / Creatine kinase B [clone CKBB/6565] (V4054)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V4054-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
|
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V4054-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V4054SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | CKBB/6565 |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | ELISA : for coating order antibody without BSA Western Blot : 2-4ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml |
| Limitations | This CKB antibody is available for research use only. |
|
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CKB antibody detects Creatine kinase B-type, also known as CKBB or brain-type creatine kinase, an enzyme that catalyzes the reversible transfer of phosphate from phosphocreatine to ADP, regenerating ATP to maintain cellular energy supply. The UniProt recommended name is Creatine kinase B-type (CKB). This cytosolic phosphotransferase belongs to the creatine kinase family, which ensures rapid ATP replenishment in cells with high and fluctuating energy demands, particularly neurons and glial cells.
CKB antibody recognizes a 43 kDa homodimeric enzyme found in brain, retina, and smooth muscle. CKB couples with mitochondrial creatine kinases to form a phosphocreatine shuttle, enabling fast energy transport from mitochondria to ATP-consuming sites such as synaptic terminals, ion pumps, and contractile fibers. In the nervous system, CKBB localizes near Na+/K+-ATPase and vesicle cycling complexes, providing an immediate ATP buffer during neurotransmission. Its expression correlates with neuronal differentiation and synaptic activity, and its release into cerebrospinal fluid or blood serves as an indicator of neural damage or ischemic stress.
Structurally, CKB forms either homodimers (CKBB) or heterodimers with muscle-type subunits (CKMB). Each subunit contains binding domains for ATP and creatine, and the enzymeâs catalytic efficiency is modulated by magnesium ions and pH. The CKB antibody is commonly used in western blot, ELISA, and immunohistochemistry to evaluate tissue-specific expression and metabolic status. In oncology, CKBB is often upregulated in cancers such as small-cell lung carcinoma, neuroblastoma, and colorectal adenocarcinoma, reflecting its role in supporting tumor energy metabolism and cell migration.
The CKB gene is located on chromosome 14q32.33 and encodes a 381-amino acid enzyme highly conserved across species. Transcription of CKB is regulated by CREB and Sp1, integrating neuronal activity and hormonal signals. Loss of Ckb expression in animal models causes impaired memory, reduced motor performance, and susceptibility to hypoxia, underscoring its neuroprotective role. Beyond the nervous system, CKB participates in sperm motility, retinal photoreceptor activity, and muscle contraction, where localized energy control is critical.
Because CKB reflects the energetic status of neural and proliferative tissues, CKB antibody is widely used for studying brain metabolism, mitochondrial function, and cancer energetics. NSJ Bioreagents offers validated reagents for human, mouse, and rat applications, optimized for immunofluorescence, immunoblotting, and immunocytochemistry.
Optimal dilution of the CKB antibody should be determined by the researcher.
A recombinant human full-length protein was used as the immunogen for the CKB antibody.
Aliquot the CKB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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