Keratin 5 Antibody KRT5/6466 / KRT5 Monoclonal Antibody [clone KRT5/6466] (V8794)

Keratin 5 Antibody KRT5/6466 by IHC in Human Prostate. Immunohistochemistry of FFPE human prostate tissue using Keratin 5 Antibody KRT5/6466. Brown DAB staining is concentrated in epithelial cells surrounding glandular structures, with a predominantly cytoplasmic distribution. The KRT5 Monoclonal Antibody highlights basal epithelial populations, while the glandular lumens and surrounding stromal tissue exhibit comparatively little staining. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before antibody incubation.

Keratin 5 Antibody KRT5/6466 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Keratin 5 Antibody KRT5/6466 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band at approximately 150 kDa, consistent with intact IgG. Under reducing conditions, distinct bands are observed at approximately 50 kDa and 25 kDa, corresponding to the expected immunoglobulin heavy and light chains, respectively. The electrophoretic profile is consistent with the expected composition of a purified mouse monoclonal antibody preparation.

Keratin 5 Antibody KRT5/6466 by Protein Microarray. Protein microarray specificity analysis of Keratin 5 Antibody KRT5/6466 using a HuProt human protein microarray containing more than 19,000 full-length proteins. The graph shows a prominent leading binding signal followed by substantially weaker signals across the remaining ranked proteins. The KRT5 Monoclonal Antibody demonstrates preferential recognition of its intended target under the assay conditions, supporting its specificity. Z-score and S-score measurements are used to assess relative binding strength and target selectivity.