CK19 Polyclonal Antibody / Cytokeratin 19 (R31920)

CK19 Polyclonal Antibody by WB in six human tissue and cell lysates. Western blot analysis using CK19 Polyclonal Antibody demonstrates immunoreactive bands at approximately 43 kDa across six human samples. Lane 1: placenta; lane 2: MCF7 breast carcinoma cells; lane 3: SW620 colorectal carcinoma cells; lane 4: COLO-320 colorectal carcinoma cells; lane 5: HepG2 hepatocellular carcinoma cells; and lane 6: PANC-1 pancreatic carcinoma cells. All six lanes exhibit bands near the expected molecular weight of cytokeratin 19, with moderately stronger signals in the first three lanes and comparatively weaker signals in lanes 4-6. The blot demonstrates KRT19 detection across multiple human epithelial cell models and placental tissue.

CK19 Polyclonal Antibody by WB in rat and mouse samples. Western blot analysis using CK19 Polyclonal Antibody demonstrates immunoreactive bands at approximately 43 kDa in rodent tissue and cell lysates. Lane 1: rat lung; lane 2: rat small intestine; lane 3: mouse lung; and lane 4: mouse HEPA1-6 hepatoma cells. All four lanes exhibit distinct bands migrating near the expected molecular weight of cytokeratin 19. Signal intensity is relatively consistent across the samples, with the rat lung band appearing slightly stronger. No prominent additional bands are visible within the displayed molecular weight range. These results demonstrate recognition of KRT19-associated proteins in both rat and mouse samples.

CK19 Polyclonal Antibody by IHC in human lung cancer. Immunohistochemistry of formalin-fixed, paraffin-embedded human lung cancer tissue using CK19 Polyclonal Antibody. Brown immunoreactivity highlights irregular epithelial tumor formations distributed among densely cellular, comparatively weakly stained regions. Several positively stained structures exhibit prominent cytoplasmic labeling, while surrounding tumor and stromal compartments show variable or limited staining. Hematoxylin counterstaining reveals nuclei in blue. The heterogeneous staining distribution illustrates KRT19 expression within selected epithelial components of the carcinoma. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 minutes, followed by cooling before staining.

CK19 Polyclonal Antibody by IHC in human lung cancer showing glandular epithelial staining. Immunohistochemistry of formalin-fixed, paraffin-embedded human lung cancer tissue using CK19 Polyclonal Antibody. Brown cytoplasmic immunoreactivity outlines irregular glandular and epithelial structures surrounding branching luminal spaces. The staining is particularly prominent along the epithelial boundaries, contrasting with adjacent densely cellular regions exhibiting comparatively limited immunoreactivity. Hematoxylin counterstaining identifies nuclei in blue. The distribution demonstrates KRT19 expression within differentiated epithelial structures of the lung carcinoma specimen. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 minutes, followed by cooling before staining.

IHC testing of FFPE human lung cancer tissue with CK19 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE mouse small intestine with CK19 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

CK19 Polyclonal Antibody by IHC in rat small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded rat small intestine tissue using CK19 Polyclonal Antibody. Brown immunoreactivity highlights numerous elongated epithelial structures extending through the intestinal mucosa, with staining concentrated along the glandular and crypt-associated epithelial compartments. The positively stained epithelial profiles contrast with the surrounding lamina propria, which exhibits comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The staining demonstrates KRT19 expression within rat intestinal epithelium and supports the antibody's documented rat reactivity. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 20 minutes, followed by cooling before staining.

CK19 Polyclonal Antibody by IF in human colon cancer. Immunofluorescence analysis of formalin-fixed, paraffin-embedded human colon cancer tissue using CK19 Polyclonal Antibody. Green fluorescence highlights irregular epithelial and glandular structures within the carcinoma, with staining concentrated in the cytoplasmic compartments of cells surrounding distinct luminal spaces. The positively stained epithelial formations contrast with adjacent regions exhibiting comparatively limited fluorescence. DAPI counterstaining identifies nuclei in blue, revealing the cellular organization of the tissue. The distribution demonstrates KRT19 expression within the neoplastic epithelial compartment. Heat-induced epitope retrieval was performed by steaming tissue sections in EDTA buffer, pH 8, for 20 minutes before immunofluorescent staining.

Immunofluorescent staining of FFPE human MCF7 cells with CK19 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

CK19 Polyclonal Antibody by FACS in human MCF7 cells. Flow cytometric analysis of human MCF7 breast carcinoma cells using CK19 Polyclonal Antibody at 1 ug per million cells following blocking with goat serum. The blue histogram represents antibody-stained cells, the green histogram represents the isotype control, and the red histogram represents cells alone. The antibody-stained population exhibits a pronounced rightward fluorescence shift relative to both control populations. The blue peak is centered near 10^4 fluorescence units, compared with approximately 10^3 for the isotype control and 10^2 for unstained cells. The separation demonstrates increased antibody-associated fluorescence in MCF7 cells under the tested conditions.