CK18 Antibody KRT18/836 / Cytokeratin 18 Monoclonal Antibody [clone KRT18/836] (V2674)

CK18 Antibody KRT18/836 by IF in human HeLa cells. Immunofluorescence staining of human HeLa cells using CK18 Antibody clone KRT18/836, detected in green, with RedDot nuclear counterstaining in red. Strong green fluorescence highlights an extensive cytoplasmic filament network surrounding the nuclei and extending throughout the cellular periphery. The staining forms a dense, interconnected pattern consistent with the organization of keratin intermediate filaments in epithelial cells. Red nuclear fluorescence provides contrast with the surrounding cytoplasmic structures. The Cytokeratin 18 Monoclonal Antibody demonstrates the expected filamentous distribution of KRT18 in this human epithelial carcinoma cell model.

CK18 Antibody KRT18/836 by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma tissue using CK18 Antibody clone KRT18/836. Strong brown cytoplasmic immunoreactivity is visible throughout neoplastic glandular epithelial structures, highlighting carcinoma cells surrounding irregular luminal spaces. The staining is concentrated within the epithelial compartment, while adjacent stromal regions exhibit comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue, providing contrast with the positively stained tumor cells. The Cytokeratin 18 Monoclonal Antibody was applied following heat-induced epitope retrieval in 10 mM Tris containing 1 mM EDTA, pH 9, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

CK18 Antibody KRT18/836 by WB in human HCT116 cells. Western blot analysis of human HCT116 colorectal carcinoma cell lysate using CK18 Antibody clone KRT18/836. A single prominent immunoreactive band is detected at approximately 40-45 kDa, migrating slightly below the predicted molecular weight of human KRT18, approximately 48 kDa. The band is clearly distinguishable against a low-background blot, with no prominent additional bands visible in the displayed molecular weight range. Molecular weight markers are provided alongside the sample lane for reference. The Cytokeratin 18 Monoclonal Antibody recognizes a protein migrating near the expected molecular weight of KRT18, supporting its use in western blot analysis of human epithelial cell lysates.

CK18 Antibody KRT18/836 by protein microarray. Protein Microarray Validated analysis of CK18 Antibody clone KRT18/836 using a HuProt human protein microarray containing more than 19,000 full-length proteins. The graph identifies KRT18 as the highest-ranking binding target, with a Z-score of approximately 54.5. The next-ranked proteins exhibit substantially lower signals, producing a clear separation between KRT18 and the remaining targets. The Cytokeratin 18 Monoclonal Antibody demonstrates strong preferential recognition of its intended antigen under the tested conditions. This result supports target selectivity in the protein microarray assay, although it does not independently establish specificity in every biological sample or experimental application.

CK18 Antibody KRT18/836 purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free CK18 Antibody clone KRT18/836 under non-reduced (NR) and reduced (R) conditions. The non-reduced preparation exhibits a prominent band near 150 kDa, consistent with intact immunoglobulin. Under reduced conditions, a prominent band is visible near 50 kDa, corresponding to the immunoglobulin heavy chain, with an additional band near 25-30 kDa representing the light chain. Molecular weight standards are included for reference. This analysis evaluates the electrophoretic characteristics and purity of the Cytokeratin 18 Monoclonal Antibody preparation rather than detection of endogenous KRT18 protein.