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Home >> Antibodies >> CK14 Antibody / Cytokeratin 14 / KRT14

CK14 Antibody / Cytokeratin 14 / KRT14 [clone KRT14/4130] (V9658)

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  Catalog No Formulation Size Price (USD)  
Image V9658-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9658-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9658SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human prostate tissue with CK14 antibody (clone KRT14/4130) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate tissue with CK14 antibody (clone KRT14/4130) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate tissue with CK14 antibody (clone KRT14/4130) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name KRT14/4130
Purity Protein A/G affinity
UniProt P02533
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CK14 antibody is available for research use only.
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Description

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells.Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors.Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Application Notes

Optimal dilution of the CK14 antibody should be determined by the researcher.

Immunogen

A portion of amino acids351-472 was used as the immunogen for the CK14 antibody.

Storage

Aliquot the CK14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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