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Home >> Antibodies >> Citrin Antibody / ARALAR2 / SLC25A13

Citrin Antibody / ARALAR2 / SLC25A13 (RQ7700)

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  Catalog No Formulation Size Price (USD)  
Image RQ7700 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human liver cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
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Immunofluorescent staining of FFPE human liver cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human colon cancer tissue with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with Citrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human diffuse large B cell lymphoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendix adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human right renal oncocytoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with Citrin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Raji, 2) human A431, 3) human 293T, 4) human HepG2, 5) rat kidney and 6) rat liver tissue lysate with Citrin antibody. Predicted molecular weight ~74 kDa.
Flow cytometry testing of human JK-1 cells with Citrin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Citrin antibody.
Flow cytometry testing of human U-87 MG cells with Citrin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Citrin antibody.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UJS0
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Citrin antibody is available for research use only.
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Description

Citrin also known as solute carrier family 25, member 13 (also called Citrin and ARALAR-related gene 2) is a protein which in humans is encoded by the SLC25A13 gene. This gene is a member of the mitochondrial carrier family. The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. The protein catalyzes the exchange of aspartate for glutamate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. Mutations in this gene result in citrullinemia, type II. Multiple transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Citrin antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-A643) was used as the immunogen for the Citrin antibody.

Storage

After reconstitution, the Citrin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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