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Home >> Antibodies >> CIP4 Antibody / TRIP10

CIP4 Antibody / TRIP10 (RQ6812)

  Catalog No Formulation Size Price (USD)  
Image RQ6812 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with CIP4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human HeLa and 3) rat testis tissue lysate with CIP4 antibody. Expected molecular weight: 40-85 kDa (multiple isoforms).
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q15642
Localization Cytoplasmic, cell membrane
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This CIP4 antibody is available for research use only.
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Description

Cdc42-interacting protein 4 is a protein that in humans is encoded by the TRIP10 gene. TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Application Notes

Optimal dilution of the CIP4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-M182) was used as the immunogen for the CIP4 antibody.

Storage

After reconstitution, the CIP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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