Chromogranin A Antibody [clone LK2H10] (V2099)

Description

Chromogranin A antibody clone LK2H10 is a monoclonal antibody that detects chromogranin A, a glycoprotein stored in neuroendocrine secretory granules. Like clone CGA/414, clone LK2H10 is widely used to identify cells and tumors with neuroendocrine differentiation. NSJ Bioreagents supplies this antibody as a trusted tool for both diagnostic pathology and neuroendocrine biology research.

Chromogranin A antibody clone LK2H10 shows strong cytoplasmic staining in neuroendocrine cells, including those in the adrenal medulla, pancreas, pituitary, and gastrointestinal tract. In pathology, it is an essential marker for diagnosing neuroendocrine tumors such as carcinoids, pheochromocytomas, and paragangliomas. The antibody consistently reveals tumor cells that retain secretory granules, providing pathologists with a sensitive and specific marker for neuroendocrine classification.

Researchers use chromogranin A antibody clone LK2H10 to investigate the role of CgA in hormone and peptide release. CgA processing produces biologically active fragments, such as catestatin, which modulate vascular tone and catecholamine secretion. Detecting CgA with clone LK2H10 allows scientists to study these pathways in endocrine regulation, stress response, and cardiovascular physiology.

Clone LK2H10 has also been employed in oncology studies, where neuroendocrine markers are used to evaluate mixed tumors and determine whether a neoplasm exhibits neuroendocrine differentiation. It performs well in formalin-fixed, paraffin-embedded tissue, frozen sections, and immunofluorescence, ensuring broad applicability. Alternate names include CgA antibody, secretory granule glycoprotein antibody, and neuroendocrine tumor marker chromogranin A antibody.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Chromogranin A antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human pheochromocytoma cells were used as the immunogen for this Chromogranin A antibody.

Storage

Store the Chromogranin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).