- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CHP1 Antibody / Calcineurin B homologous protein 1 (FY13335)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13335 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
CHP1 antibody detects Calcineurin B homologous protein 1, a calcium-binding regulatory protein encoded by the CHP1 gene located on chromosome 15q15.1. CHP1 is a cytoplasmic and membrane-associated protein expressed in a wide range of tissues, including brain, heart, kidney, and skeletal muscle. It belongs to the EF-hand superfamily of calcium-binding proteins and functions as an essential regulator of sodium-hydrogen exchanger (NHE) activity, vesicle trafficking, and signal transduction. CHP1 acts as a molecular sensor of intracellular calcium levels, modulating transporter activity and contributing to pH homeostasis and cellular excitability.
CHP1 directly binds to the cytoplasmic tails of NHE1, NHE2, and NHE3 isoforms, stabilizing their conformation and enhancing plasma membrane localization. This regulatory role links calcium signaling to intracellular pH regulation and ion balance. CHP1 also associates with calcineurin and other phosphatases, influencing downstream signaling in calcium-dependent pathways. High expression in neurons and cardiac cells underscores its importance in excitable tissues where calcium fluctuations tightly control function and metabolism.
Structurally, CHP1 contains four EF-hand motifs, three of which bind calcium ions, and a myristoylation site that facilitates membrane association. Calcium binding induces conformational changes that alter its interaction with target proteins, providing a molecular mechanism for activity regulation. CHP1 belongs to the calcineurin B homologous protein family, which includes CHP2 and CHP3, all serving as modulators of NHEs and other transporters. CHP1 also co-localizes with NHE1 at the plasma membrane and Golgi apparatus, supporting its dual role in membrane trafficking and ion transport.
Functionally, CHP1 contributes to multiple cellular processes including cytoskeletal organization, vesicle transport, and cell migration. It stabilizes the actin cytoskeleton through NHE1ERM protein interactions and participates in cellular stress adaptation. CHP1 is implicated in the MAPK and calcium signaling pathways that mediate growth, differentiation, and apoptosis. In neurons, CHP1 interacts with voltage-gated ion channels and regulates excitatory signaling, while in cardiomyocytes it influences contractility by fine-tuning calcium dynamics.
Dysregulation of CHP1 has been linked to neuropathies, cardiac dysfunction, and cancer. Mutations in CHP1 cause autosomal recessive ataxia, characterized by impaired motor coordination and Purkinje cell loss. Abnormal CHP1 expression can also contribute to altered NHE1 activity in glioblastoma and other tumors, affecting intracellular pH and invasive potential. Pathway analysis places CHP1 in ion transport regulation, calcium signaling, and cellular stress adaptation mechanisms.
Immunohistochemical staining using CHP1 antibody shows cytoplasmic and membrane localization, with prominent expression in neurons and cardiac muscle. CHP1 antibody from NSJ Bioreagents provides a powerful reagent for studying calcium signaling, ion transport regulation, and neurological or cardiac disease mechanisms.
Optimal dilution of the CHP1 antibody should be determined by the researcher.
A synthetic peptide corresponding to a sequence at the N-terminus of human CHP1 was used as the immunogen for the CHP1 antibody.
After reconstitution, the CHP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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