CHCHD6 Antibody / MICOS complex subunit MIC25 Antibody (FY13625)

CHCHD6 Antibody by IF in U2OS cells. Immunofluorescence analysis using MICOS complex subunit MIC25 Antibody reveals green punctate and perinuclear cytoplasmic staining in U2OS cells, alongside red alpha-tubulin labeling and blue nuclear counterstaining. Cells were incubated with 5 ug/mL primary antibody overnight at 4oC and detected using fluorophore-conjugated secondary antibodies. The pattern is compatible with mitochondrial-associated distribution, but the image does not include a mitochondrial colocalization marker.

CHCHD6 Antibody by FACS in Jurkat cells. Flow cytometry analysis using MICOS complex subunit MIC25 Antibody demonstrates a rightward fluorescence shift in fixed, permeabilized Jurkat cells (blue) compared with the rabbit IgG isotype control (green) and unstained blank (red). Intracellular staining used 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC, followed by fluorescent secondary antibody. The experiment measures intracellular rather than cell-surface CHCHD6.

CHCHD6 Antibody by WB in human cells and rodent testis. Western blot analysis using MICOS complex subunit MIC25 Antibody detects bands consistent with the expected approximately 26 kDa molecular weight in human A431 (lane 1), Jurkat (lane 2), rat testis (lane 3), and mouse testis (lane 4) lysates. Reduced samples containing 30 ug total protein per lane were resolved by 12% SDS-PAGE and transferred to nitrocellulose. The primary antibody was applied at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection. Additional higher bands are visible in the rodent samples but have not been assigned identities.