CHCHD4 Antibody / Mitochondrial intermembrane space import and assembly protein 40 Antibody (FY13591)

CHCHD4 Antibody by IF in A549 cells. Immunofluorescence staining using Mitochondrial intermembrane space import and assembly protein 40 Antibody reveals green signal in the cytoplasm of A549 cells, with brighter punctate and perinuclear regions and DAPI-stained blue nuclei. The observed distribution is compatible with a mitochondrial-associated protein, although organelle-specific colocalization was not tested in this image. Cells underwent enzyme-mediated antigen retrieval for 15 minutes, followed by blocking in 10% goat serum. Primary antibody was applied at 5 ug/mL overnight at 4oC, followed by Fluoro488-conjugated secondary antibody at 1:500.

CHCHD4 Antibody by IHC in human liver carcinoma. Immunohistochemistry of paraffin-embedded human liver carcinoma using Mitochondrial intermembrane space import and assembly protein 40 Antibody shows widespread granular brown staining in tumor cells, including cytoplasmic regions. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.

CHCHD4 Antibody by WB in human and rodent samples. Western blot analysis using Mitochondrial intermembrane space import and assembly protein 40 Antibody detects an immunoreactive band near 22 kDa in human Daudi (lane 1), Ramos (lane 2), Raji (lane 3), 293T (lane 4), rat liver (lane 5), and mouse liver (lane 6). The calculated molecular weight is approximately 16 kDa. Reduced lysates containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.