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Home >> Antibodies >> CH25H Antibody (N-Terminal Region)

CH25H Antibody (N-Terminal Region) (F54079)

  Catalog No Formulation Size Price (USD)  
Image F54079-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 429
Image
F54079-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 199
Bulk quote request
Western blot testing of CH25H antibody at 1:1000: Lane 1) human brain lysate, 2) mouse heart lysate and 3) mouse lung lysate. Predicted molecular weight ~32 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Pig
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O95992
Applications Western Blot : 1:1000-1:2000
Limitations This CH25H antibody is available for research use only.
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Description

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25- hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the CH25H antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 47-76 from human CH25H was used as the immunogen for the CH25H antibody.

Storage

Aliquot the CH25H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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