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Home >> Antibodies >> cGAS Antibody / cGAMP Synthase

cGAS Antibody / cGAMP Synthase (RQ6412)

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  Catalog No Formulation Size Price (USD)  
Image RQ6412 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human Caco-2 cells with cGAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human Caco-2 cells with cGAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human A431, 4) rat lung and 5) mouse RAW264.7 cell lysate with cGAS antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of human U937 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
Flow cytometry testing of human ThP-1 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q8N884
Localization Cytoplasmic, nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This cGAS antibody is available for research use only.
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Description

cGAS (cyclic GMP-AMP synthase), also known as MB21D1 (Mab-21 domain containing 1), h-cGAS or C6orf150, is a 522 amino acid cytoplasmic nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAS is suggested to have antiviral activity by acting as a key cytosolic DNA sensor. cGAS binds to cytosolic DNA, which leads to cGAMP synthesis and activation of TMEM173, thereby trigger type-I interferon production. Expressed in monocytic cell line THP1, cGAS exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 6q13.

Application Notes

Optimal dilution of the cGAS antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids L208-F522) was used as the immunogen for the cGAS antibody.

Storage

After reconstitution, the cGAS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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