cGAS Antibody / Cytosolic DNA Sensor Antibody (FY13465)

cGAS Antibody / Cytosolic DNA Sensor Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using cGAS Antibody / Cytosolic DNA Sensor Antibody. cGAS expression is detected predominantly within the cytoplasm of tumor cells, consistent with its role as an innate immune DNA sensor that recognizes cytosolic double-stranded DNA and activates the cGAS-STING signaling pathway. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

cGAS Antibody / Cytosolic DNA Sensor Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using cGAS Antibody / Cytosolic DNA Sensor Antibody. cGAS expression is detected predominantly within the cytoplasm of tumor cells, consistent with its function as an innate immune DNA sensor that recognizes cytosolic double-stranded DNA and initiates cGAS-STING signaling. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by a peroxidase-conjugated goat anti-rabbit IgG secondary antibody. Immunoreactivity was visualized using an HRP Super Vision detection system with DAB as the chromogen.

cGAS Antibody / Cytosolic DNA Sensor Antibody Human Cell WB. Western blot analysis of 1) human THP-1, 2) human A431, 3) human CACO-2, and 4) human SiHa cell lysates using cGAS Antibody / Cytosolic DNA Sensor Antibody. A specific immunoreactive band is detected at approximately 59-65 kDa in all tested cell lines, consistent with cGAS expression. The predicted molecular weight of cGAS is approximately 58 kDa. These results demonstrate reliable detection of cGAS, a cytosolic DNA sensor that activates the cGAS-STING signaling pathway to initiate innate immune responses, type I interferon production, and inflammatory signaling following recognition of cytoplasmic double-stranded DNA.

cGAS Antibody / Cytosolic DNA Sensor Antibody Mouse WB. Western blot analysis of 1) mouse thymus tissue and 2) mouse RAW264.7 cell lysates using cGAS Antibody / Cytosolic DNA Sensor Antibody. A specific immunoreactive band is detected at approximately 59-65 kDa in both samples, consistent with cGAS expression. The predicted molecular weight of cGAS is approximately 58 kDa. These results demonstrate reliable detection of cGAS in mouse immune tissues and macrophage-derived cells, supporting its established role as a cytosolic DNA sensor that activates the cGAS-STING signaling pathway during innate immune and inflammatory responses.