cGAS Antibody / cGAMP Synthase (RQ6412)

Immunofluorescent staining of FFPE human Caco-2 cells with cGAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HeLa, 2) Caco-2 and 3) A431 cell lysate with cGAS antibody. Predicted molecular weight ~59 kDa.

Flow cytometry testing of human U937 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.

Flow cytometry testing of human ThP-1 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.

cGAS Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue with cGAS antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. cGAS immunoreactivity is observed predominantly within the cytoplasm of scattered lymphoid cells throughout the tonsil, consistent with the expected localization of this innate immune DNA sensor involved in activation of the cGAS-STING signaling pathway. Cell nuclei are counterstained with hematoxylin (blue).

cGAS Antibody Human HeLa WT vs cGAS Knockout WB. Western blot testing of 1) human HeLa wild-type and 2) human HeLa cGAS knockout whole cell lysates with cGAS antibody at 0.5 ug/ml. A specific band is detected at approximately 59 kDa in the wild-type lysate but is absent in the CGAS knockout lysate, confirming the specificity of the antibody for endogenous cGAS. GAPDH serves as the loading control and demonstrates comparable protein loading between samples. The observed molecular weight is consistent with the predicted molecular weight of approximately 59 kDa.