Ceruloplasmin Antibody / Iron Metabolism Protein Antibody (R31856)

Ceruloplasmin Antibody HepG2 Cell FACS. Flow cytometric analysis of fixed human HepG2 cells using Ceruloplasmin Antibody / Iron Metabolism Protein Antibody demonstrates a pronounced rightward shift of the Ceruloplasmin-positive population (blue) relative to both the isotype control (green) and unstained cells (red), consistent with expression of Ceruloplasmin (CP). Ceruloplasmin is a multicopper ferroxidase that plays a central role in iron metabolism, copper transport, and maintenance of metal ion homeostasis. By catalyzing the oxidation of ferrous iron to ferric iron, Ceruloplasmin facilitates iron transport and utilization while contributing to systemic mineral balance and cellular metabolic regulation. The observed fluorescence shift supports the utility of this antibody for flow cytometric detection of Ceruloplasmin expression. Red=cells alone; Green=isotype control; Blue=Ceruloplasmin antibody.

Ceruloplasmin Antibody HepG2 Western Blot. Western blot analysis of human HepG2 cell lysate using Ceruloplasmin Antibody / Iron Metabolism Protein Antibody demonstrates a distinct band at approximately 130-140 kDa, consistent with expression of Ceruloplasmin (CP). Ceruloplasmin is a multicopper ferroxidase that plays a central role in iron metabolism, copper transport, and maintenance of metal ion homeostasis. By catalyzing the oxidation of ferrous iron to ferric iron, Ceruloplasmin facilitates iron transport and utilization while contributing to systemic mineral balance and cellular metabolic regulation. The observed molecular weight is consistent with the heavily glycosylated nature of Ceruloplasmin, which commonly migrates above its predicted molecular weight of approximately 122 kDa during SDS-PAGE analysis.