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Home >> Antibodies >> CEN1 Antibody / Centrin 1

CEN1 Antibody / Centrin 1 [clone CETN1/9877] (V5655)

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  Catalog No Formulation Size Price (USD)  
Image V5655-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5655-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5655SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human testis tissue with CEN1 antibody (clone CETN1/9877). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human testis tissue with CEN1 antibody (clone CETN1/9877). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CEN1 antibody (clone CETN1/9877) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name CETN1/9877
Purity Protein A/G affinity
UniProt Q12798
Localization Cell projection, Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CEN1 antibody is available for research use only.
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Description

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Tissues where cilia are present, such as the retina and testis, express both Centrin-1 and -2, but Centrin-2 is also expressed in nondifferentiated, nonciliated retinal cells (retinoblastoma cells), liver, skeletal muscle, and cardiac muscle. In these tissues, Centrin associates with the centrosomes, mitotic spindle poles, and basal bodies. Knockdown studies reveal a requirement for Centrin in centriole duplication and organization of spindle pole morphology and the completion of cytokinesis. Centrin-3 plays a role in centrosome reproduction.

Application Notes

Optimal dilution of the CEN1 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 1-172 from human CETN1 protein was used as the immunogen for the CEN1 antibody.

Storage

Aliquot the CEN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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