- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CELA3B Antibody for IHC / Chymotrypsin like elastase 3B [clone MSVA-410M] (V6076)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V6076-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 654.50 | |
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V6076-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | MSVA-410M |
| UniProt | P08861 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This CELA3B/Chymotrypsin like elastase 3B antibody is available for research use only. |
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CELA3B Antibody for IHC recognizes Chymotrypsin-like elastase family member 3B, a secreted digestive serine protease commonly referred to as pancreatic elastase 3B or elastase 3B. The human CELA3B gene is located on chromosome 1p36.12 and encodes a protein that is highly expressed in pancreatic acinar cells, where it is synthesized, processed, and stored within zymogen granules prior to secretion into the duodenum. As a member of the chymotrypsin family of serine proteases, CELA3B plays an important role in dietary protein digestion through regulated proteolytic activity in the intestinal lumen.
Chymotrypsin-like elastase family member 3B belongs to the elastase subfamily of trypsin-like proteases and shares strong structural homology with CELA3A. The enzyme is produced as a preproprotein containing a signal peptide that directs entry into the secretory pathway, followed by an activation peptide that maintains the protease in an inactive state until cleavage by trypsin. The mature enzyme adopts the conserved serine protease fold with a catalytic triad that mediates peptide bond hydrolysis. Because of its secretory routing, CELA3B localizes to the endoplasmic reticulum and Golgi during biosynthesis and is concentrated within cytoplasmic secretory granules of pancreatic acinar cells prior to extracellular release.
CELA3B is sometimes referenced in clinical literature under the term fecal elastase 1, reflecting its stability in stool and its use as a biochemical marker of pancreatic exocrine function. In tissue-based research settings, elastase 3B is widely recognized as a lineage-associated marker of acinar differentiation. Immunohistochemical studies have demonstrated strong expression in normal pancreatic acinar cells, while comparative tumor profiling supports its relevance in the evaluation of pancreatic acinar cell carcinoma and other exocrine pancreatic neoplasms. These characteristics make CELA3B a useful target in research panels focused on pancreatic development, differentiation, and tumor classification.
Genetic investigations have also linked CELA3B variants to inherited or familial pancreatitis phenotypes, underscoring its biological importance in digestive enzyme regulation and pancreatic homeostasis. Dysregulation of digestive protease activation is a central mechanism in pancreatitis pathogenesis, and CELA3B contributes to the tightly controlled proteolytic environment of the exocrine pancreas. The recombinant mouse monoclonal clone MSVA-410M is designed to detect CELA3B expression in formalin-fixed, paraffin-embedded tissue samples, supporting research applications centered on pancreatic acinar biology and elastase family protein expression.
1. Optimal dilution of the CELA3B antibody for IHC should be determined by the researcher.
2. This CELA3B/Chymotrypsin like elastase 3B antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Recombinant human CELA3B protein fragment (around amino acids 82-238) (exact sequence is proprietary) was used as the immunogen for the CELA3B antibody for IHC.
CELA3B/Chymotrypsin like elastase 3B antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
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