CELA3B Antibody - Microarray Validated / Elastase 3B [clone CELA3B/1757] (V3449)

Immunohistochemistry of CELA3B antibody (microarray validated clone CELA3B/1757) in human pancreas. Formalin-fixed, paraffin-embedded human pancreatic tissue demonstrates strong granular cytoplasmic staining in acinar cells, consistent with the secretory localization of Chymotrypsin-like elastase family member 3B, while surrounding stromal and ductal structures show minimal staining. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free CELA3B Antibody (clone CELA3B/1757). Confirmation of Integrity and Purity of the Antibody.

Protein microarray specificity validation of CELA3B antibody (clone CELA3B/1757). Analysis was performed using the HuProt(TM) microarray containing more than 19,000 full-length human proteins. CELA3B was identified as the top-ranked target, demonstrating strong signal intensity relative to all other proteins on the array and supporting the high specificity of this microarray validated monoclonal antibody.
Z-score represents the strength of the signal generated when the antibody, in combination with a fluorescently tagged anti-IgG secondary antibody, binds to a specific protein on the HuProt(TM) array. Z-scores are expressed in standard deviations above the mean signal of all proteins on the array. Proteins are ranked in descending order based on Z-score. The S-score is defined as the difference in Z-scores between adjacent ranked proteins and reflects the relative target specificity of the antibody for its intended antigen.