CEACAM1 Antibody / BGP-1 / CD66a [clone CEACAM1/12659] (V5792)

Western blot analysis of CEACAM1 antibody in human SK-MEL-28 cell lysate. A strong high molecular weight band and broad signal are observed well above the predicted molecular weight of 58 kDa. In some experimental settings, a lighter band may be detected near the predicted molecular weight, representing less glycosylated forms of CEACAM1. The predicted molecular weight of CEACAM1, also known as BGP-1, is approximately 58 kDa based on amino acid sequence; however, CEACAM1 is a heavily N-glycosylated type I membrane glycoprotein. Extensive N-linked glycosylation, heterogeneous glycoform distribution, and potential dimerization can result in markedly slower SDS-PAGE migration and apparent molecular weights substantially higher than the sequence-predicted size. The prominent high molecular weight signal observed here is therefore consistent with glycosylated CEACAM1 species in melanoma-derived cells.

Immunohistochemistry analysis of CEACAM1 antibody in human colon tissue. FFPE human colon sections show membranous and apical HRP-DAB brown staining in glandular epithelial cells, consistent with CEACAM1 expression at the luminal surface. Stromal elements show minimal staining. The inset negative control, in which PBS was used in place of primary antibody, shows no specific brown chromogenic signal. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free CEACAM1 antibody (clone CEACAM1/12659) as confirmation of integrity and purity.