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Home >> Antibodies >> CDX2 Antibody

CDX2 Antibody [clone CDX2/1690] (V3415)

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  Catalog No Formulation Size Price (USD)  
Image V3415-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3415-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3415SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
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IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/1690). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free CDX2 Antibody (clone CDX2/1690). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CDX2/1690
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt Q99626
Localization Nuclear
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This CDX2 antibody is available for research use only.
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Description

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoids and is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDX2 antibody to be titered up or down for optimal performance.

Immunogen

Amino acids 150-249 from the human protein were used as the immunogen for this CDX2 antibody.

Storage

Store the CDX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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