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Home >> Antibodies >> CDT1 Antibody / DNA replication factor Cdt1

CDT1 Antibody / DNA replication factor Cdt1 (RQ7910)

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  Catalog No Formulation Size Price (USD)  
Image RQ7910 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human HepG2 cells with CDT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDT1 antibody.
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Flow cytometry testing of human HepG2 cells with CDT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDT1 antibody.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Raji, 4) human K562, 5) rat liver and 6) mouse liver tissue lysate with CDT1 antibody. Predicted molecular weight ~60 kDa, commonly observed at 60-70 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H211
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This CDT1 antibody is available for research use only.
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Description

CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene. The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.

Application Notes

Optimal dilution of the CDT1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids P18-L546) was used as the immunogen for the CDT1 antibody.

Storage

After reconstitution, the CDT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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