CDT1 Antibody / DNA Replication Licensing Factor Antibody (RQ6763)

CDT1 Antibody HepG2 Flow Cytometry. Flow cytometric analysis of human HepG2 cells using CDT1 antibody demonstrates a clear rightward shift relative to the isotype control population, consistent with expression of Chromatin Licensing and DNA Replication Factor 1 (CDT1). CDT1 is a DNA replication licensing factor that regulates assembly of the pre-replication complex and ensures accurate genome duplication during cell cycle progression. The observed staining pattern supports detection of endogenous CDT1 expression and demonstrates the utility of this antibody for studies of DNA replication licensing, cellular proliferation, and genome stability pathways. Cells were stained with 1 ug antibody per million cells following blocking with goat sera.

CDT1 Antibody Multi-Species WB. Western blot analysis of human Jurkat, HepG2, Raji, and K562 cell lysates together with rat brain, rat liver, mouse brain, and mouse liver tissue lysates using CDT1 antibody demonstrates detection of a major band at approximately 60-70 kDa, consistent with the expected molecular weight of Chromatin Licensing and DNA Replication Factor 1 (CDT1). CDT1 is a DNA replication licensing factor that functions during the G1 phase of the cell cycle to establish replication competence and ensure accurate genome duplication. The observed expression across multiple human, rat, and mouse samples supports the utility of this antibody for investigating DNA replication licensing, cell cycle regulation, cellular proliferation, and genome stability pathways. Predicted molecular weight: ~60 kDa, commonly observed at 60-70 kDa.

CDT1 Antibody Human, Rat and Mouse WB. Western blot analysis of human HepG2, HeLa, and 293T cell lysates together with rat liver, rat brain, mouse liver, and mouse brain tissue lysates using CDT1 antibody demonstrates detection of a prominent band at approximately 60-70 kDa, consistent with the expected molecular weight of Chromatin Licensing and DNA Replication Factor 1 (CDT1). CDT1 is a DNA replication licensing factor that plays an essential role in pre-replication complex assembly and regulation of genome duplication during cell cycle progression. The observed expression across multiple human, rat, and mouse samples supports the utility of this antibody for studies of DNA replication licensing, cellular proliferation, cell cycle control, and genome stability mechanisms. Predicted molecular weight: ~60 kDa, commonly observed at 60-70 kDa.

CDT1 Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells using CDT1 antibody demonstrates predominantly nuclear green fluorescence, consistent with the expected localization of Chromatin Licensing and DNA Replication Factor 1 (CDT1). CDT1 is a DNA replication licensing factor that functions in assembly of the pre-replication complex and regulation of genome duplication during cell cycle progression. Co-staining with Beta Tubulin (red) highlights the cytoskeletal network and provides contrast to the nuclear distribution of CDT1. The observed staining pattern is consistent with the established role of CDT1 in DNA replication licensing, cellular proliferation, and genome stability pathways. HIER: steam section in pH6 citrate buffer for 20 min.