CDKN1A Antibody / Cyclin-Dependent Kinase Inhibitor 1 Antibody (RQ8115)

CDKN1A Antibody by IF in Human HeLa Cells. Immunofluorescent staining of FFPE human HeLa cells using Cyclin-Dependent Kinase Inhibitor 1 Antibody (green) and a beta-tubulin monoclonal antibody (red). CDKN1A immunoreactivity is predominantly nuclear, with variable staining intensity among cells. Beta-tubulin staining highlights the cytoplasmic microtubule network. Heat-induced epitope retrieval was performed by steaming in citrate buffer (pH 6) for 20 minutes.

CDKN1A Antibody by IHC in Human Ovarian Cancer. Immunohistochemical staining of FFPE human ovarian carcinoma tissue using Cyclin-Dependent Kinase Inhibitor 1 Antibody. Scattered tumor cells exhibit brown immunoreactivity, with staining observed in both nuclear and cytoplasmic regions. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8) for 20 minutes.

CDKN1A Antibody by IHC in Human Thyroid Cancer. Immunohistochemical staining of FFPE human thyroid carcinoma tissue using Cyclin-Dependent Kinase Inhibitor 1 Antibody. Brown immunoreactivity is observed in subsets of tumor cells, with nuclear staining and variable cytoplasmic staining. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed in EDTA buffer (pH 8) for 20 minutes.

CDKN1A Antibody by WB in Human Cell Lines. Western blot analysis of human cell lysates using Cyclin-Dependent Kinase Inhibitor 1 Antibody. Lanes: 1) HeLa, 2) HepG2, 3) MCF7, 4) U-2 OS, and 5) SiHa. A band is detected at approximately 21 kDa in all five lysates, with varying signal intensity. The calculated molecular weight of CDKN1A is approximately 18 kDa, while the protein commonly migrates near 21 kDa by SDS-PAGE.