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Home >> Antibodies >> Cdk9 Antibody

Cdk9 Antibody (R32122)

  Catalog No Formulation Size Price (USD)  
Image R32122 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of human Jurkat lysate with Cdk9 antibody. Predicted/observed molecular weight ~43 kDa.
IHC testing of FFPE human intestinal cancer with Cdk9 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat intestine with Cdk9 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P50750
Localization Nuclear and cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Cdk9 antibody is available for research use only.
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Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Application Notes

Optimal dilution of the Cdk9 antibody should be determined by the researcher.

Immunogen

Amino acids 141-372 of human Cdk9 were used as the immunogen for the Cdk9 antibody.

Storage

After reconstitution, the Cdk9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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