CDK5 Antibody / Neuronal Protein Kinase Antibody (R30624)

CDK5 Antibody HeLa Cell IF. Immunofluorescent staining of HeLa cells with CDK5 antibody (green) and Alpha Tubulin antibody (red). Enzymatic antigen retrieval was performed for 15 minutes prior to staining. CDK5 immunoreactivity is observed in both the cytoplasm and nucleus, consistent with the expected localization of Cyclin Dependent Kinase 5 (CDK5), a neuronal protein kinase that regulates protein phosphorylation, cytoskeletal organization, intracellular signaling, and neuronal development. Alpha Tubulin (red) serves as a cytoskeletal marker to illustrate cellular morphology.

CDK5 Antibody Human and Rodent Brain WB. Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) rat brain, and 4) mouse brain lysates with CDK5 antibody at 0.5 ug/ml. A distinct immunoreactive band is detected at approximately 33 kDa in all samples, consistent with the predicted molecular weight of Cyclin Dependent Kinase 5 (CDK5). The results demonstrate cross-species detection of this neuronal protein kinase, which regulates protein phosphorylation, cytoskeletal organization, neuronal development, synaptic plasticity, and intracellular signaling.

CDK5 Antibody Jurkat Cell FACS. Flow cytometric analysis of fixed and permeabilized human Jurkat cells stained with CDK5 antibody. Red: blank control. Green: rabbit IgG isotype control. Blue: CDK5 antibody. The rightward shift of the antibody-stained population relative to the isotype control demonstrates specific intracellular detection of Cyclin Dependent Kinase 5 (CDK5), a neuronal protein kinase that regulates protein phosphorylation, cytoskeletal organization, intracellular signaling, neuronal development, and synaptic plasticity.