CDK4 Antibody [clone CDK4/8210R] (V4307)

CDK4 Antibody K-562 WB. Western blot analysis of Cyclin-dependent kinase 4 (CDK4) expression in K-562 cell lysate using CDK4 antibody clone CDK4/8210R. Lane 1: K-562 cell lysate. A band is detected at approximately 33-34 kDa, consistent with the predicted molecular weight of CDK4, a key regulator of G1 to S phase cell cycle progression. A faint lower-molecular-weight band is also observed below 20 kDa, which may represent proteolytic processing or non-specific reactivity.

CDK4 Antibody Human Tonsil Tissue IHC. Immunohistochemistry staining of FFPE human tonsil tissue with CDK4 antibody (clone CDK4/8210R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CDK4 antibody (CDK4/8210R) as confirmation of integrity and purity.

CDK4 Antibody Cell Line WB. Western blot analysis of Cyclin-dependent kinase 4 (CDK4) expression in cell lysates using CDK4 antibody. Lane 1: MCF7 cell lysate, Lane 2: HEK293T cell lysate. A band is detected at approximately 33–34 kDa, consistent with the predicted molecular weight of CDK4, a key regulator of G1 to S phase cell cycle progression. A lower-molecular-weight band is also observed at approximately 15–20 kDa, which may represent proteolytic processing or non-specific reactivity. The presence of CDK4 signal in both cell lines aligns with its known expression in proliferating cells.