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Home >> Antibodies >> CDK1 Antibody

CDK1 Antibody [clone 2G11] (RQ5499)

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  Catalog No Formulation Size Price (USD)  
Image RQ5499 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-2 OS cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
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Flow cytometry testing of human U-2 OS cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
Flow cytometry testing of human PC-3 cells with CDK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDK1 antibody.
Western blot testing of human 1) HEK293, 2) A549, 3) HepG2, 4) ThP-1, 5) PANC-1, 6) SW620, 7) rat RH30 and 8) mouse NIH3T3 lysate with CDK1 antibody. Predicted molecular weight ~33 kDa.
IHC staining of FFPE human colon cancer with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis with CDK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 2G11
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P06493
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This CDK1 antibody is available for research use only.
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Description

CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1). CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

Application Notes

Optimal dilution of the CDK1 antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids L66-M297) was used as the immunogen for the CDK1 antibody.

Storage

After reconstitution, the CDK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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