CDH17 Antibody Rabbit Polyclonal / Cadherin 17 (RQ6713)

Immunohistochemistry of CDH17 antibody in mouse colon tissue. The rabbit polyclonal antibody demonstrates strong membranous HRP-DAB brown staining along the lateral borders of colonic epithelial cells, consistent with Cadherin 17 localization in intestinal epithelium. Staining highlights well-defined crypt and villus structures with preserved epithelial polarity, while underlying stromal tissue shows minimal background signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to incubation.

IHC staining of FFPE rat colon tissue with CDH17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemistry of CDH17 antibody in human rectal cancer tissue. The rabbit polyclonal antibody demonstrates strong membranous HRP-DAB brown staining in malignant gland-forming epithelial cells, consistent with Cadherin 17 expression in intestinal-type adenocarcinoma. Staining highlights cohesive tumor cell membranes with preserved glandular architecture, while adjacent stromal components show minimal background signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to incubation.

Immunofluorescence of CDH17 antibody in human intestinal cancer tissue. The rabbit polyclonal antibody shows strong membranous red fluorescence localized to malignant epithelial cell borders, consistent with Cadherin 17 expression in intestinal-type tumor cells. Signal outlines gland-forming structures and highlights preserved epithelial polarity within tumor regions. Nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 minutes followed by cooling prior to incubation.

Immunofluorescent staining of FFPE human intestinal cancer tissue with CDH17 antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescence of CDH17 antibody in human Caco-2 cells. The rabbit polyclonal antibody demonstrates membranous green fluorescence outlining epithelial cell borders, consistent with Cadherin 17 localization at cell-cell junctions. Signal highlights peripheral membrane staining with minimal nuclear background. Nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to antibody incubation.

Flow cytometry testing of fixed and permeabilized human Caco-2 cells with CDH17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDH17 antibody.