CDH17 Antibody Rabbit Polyclonal / Cadherin 17 (RQ6713)

CDH17 Antibody Mouse Colon IHC. Immunohistochemistry staining of FFPE mouse colon tissue using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates strong membranous HRP-DAB brown staining along the lateral borders of epithelial cells lining intestinal villi and crypt-like glandular structures. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule that contributes to epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions within the gastrointestinal tract. The prominent membrane-associated staining highlights preserved epithelial polarity and well-organized intestinal architecture, while underlying stromal tissues exhibit minimal background reactivity. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to immunostaining.

CDH17 Antibody Rat Colon IHC. Immunohistochemistry staining of FFPE rat colon tissue using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates distinct membranous HRP-DAB brown staining within colonic epithelial cells lining glandular and crypt structures. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule involved in epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions throughout the gastrointestinal tract. CDH17 is highly expressed in intestinal epithelial tissues and serves as a well-established marker of gastrointestinal epithelial differentiation and lineage. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to immunostaining.

CDH17 Antibody Rectal Cancer IHC. Immunohistochemistry staining of FFPE human rectal adenocarcinoma tissue using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates strong membranous HRP-DAB brown staining in malignant gland-forming epithelial cells. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule involved in epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions within the gastrointestinal tract. Prominent membrane-associated staining highlights cohesive tumor cell populations and preserved glandular architecture, while adjacent stromal elements show minimal background reactivity. CDH17 is widely used as a marker of gastrointestinal epithelial differentiation and intestinal-type adenocarcinomas. HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to immunostaining.

CDH17 Antibody Intestinal Cancer IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates strong membrane-associated red fluorescence outlining malignant epithelial cells and gland-forming tumor structures. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule involved in epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions within the gastrointestinal tract. Prominent membranous labeling highlights preserved epithelial polarity and cohesive tumor cell architecture characteristic of intestinal-type adenocarcinoma. Nuclei are counterstained with DAPI (blue). HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to immunofluorescent staining.

CDH17 Antibody Epithelial Cell Border IF. Immunofluorescent staining of FFPE human intestinal cancer tissue using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates strong membrane-associated red fluorescence outlining epithelial cell borders throughout gland-forming tumor structures. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule that mediates epithelial cell-cell adhesion and contributes to maintenance of tissue architecture within the gastrointestinal tract. Prominent membrane localization highlights cohesive epithelial organization and preserved cellular polarity within malignant glandular structures. Nuclei are counterstained with DAPI (blue). HIER was performed by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling prior to immunofluorescent staining.

CDH17 Antibody Cell Junction IF. Immunofluorescent staining of human Caco-2 cells using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates prominent membrane-associated green fluorescence outlining epithelial cell borders and sites of cell-cell contact. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule that contributes to epithelial organization, maintenance of tissue integrity, and regulation of intercellular adhesion within gastrointestinal epithelia. Strong peripheral membrane staining highlights the localization of CDH17 at epithelial junctional interfaces, while nuclei are counterstained with DAPI (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunofluorescent staining.

CDH17 Antibody FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 cells using CDH17 Antibody / Liver-Intestine Cadherin Antibody demonstrates a pronounced rightward shift of the antibody-stained population (blue) relative to both the isotype control (green) and unstained cells (red), consistent with robust CDH17 expression. Cadherin 17 (LI-cadherin) is a calcium-dependent cell adhesion molecule predominantly expressed in gastrointestinal epithelial cells, where it contributes to epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions. The observed staining pattern supports detection of CDH17 in human intestinal epithelial-derived cells by flow cytometry. Cells were fixed, permeabilized, and stained with CDH17 antibody at 1 ug/million cells following blocking with goat sera. Red = cells alone, Green = isotype control, Blue = CDH17 antibody.