CDC27 Antibody | RQ6123 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ6123	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human gastric cancer with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human melanoma with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin cancer with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human rectal cancer with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder cancer with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse intestine with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestine with CDC27 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A431 cells with CDC27 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) Raji, 2) Jurkat, 3) Jurkat and 4) mouse thymus lysate with CDC27 antibody. Predicted molecular weight ~92 kDa.

Flow cytometry testing of human A431 cells with CDC27 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDC27 antibody.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt	P30260
Localization	Nuclear, cytoplasmic
Applications	Western blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This CDC27 antibody is available for research use only.
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Description

Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.

Application Notes

Optimal dilution of the CDC27 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids H609-F824) was used as the immunogen for the CDC27 antibody.

Storage

After reconstitution, the CDC27 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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CDC27 Antibody (RQ6123)

Description

Application Notes

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Storage

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Area of Research

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Protocols

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