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Home >> Antibodies >> CD99 Antibody Cocktail

CD99 Antibody Cocktail [clone 12E7 + MIC2/877] (V7952)

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  Catalog No Formulation Size Price (USD)  
Image V7952-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7952-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7952SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human Ewing's sarcoma with CD99 antibody (clones 12E7 + MIC2/877). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human Ewing's sarcoma with CD99 antibody (clones 12E7 + MIC2/877). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa + Mouse IgG1, kappa
Clone Name 12E7 + MIC2/877
Purity Protein G affinity chromatography
UniProt P14209
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This CD99 antibody is available for research use only.
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Description

Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

Application Notes

Optimal dilution of the CD99 antibody should be determined by the researcher.

Immunogen

Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) were used as the immunogen for the CD99 antibody cocktail.

Storage

Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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