CD8a Antibody [clone SPM548] (V2386)

Description

CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked heterodimer or homodimer. CD8 is expressed on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells.

Application Notes

Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human CD8 recombinant protein was used as immunogen for this CD8a antibody.

Storage

CD8a antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.