- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
CD86 Antibody [clone SPM600] (V2989)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V2989-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V2989-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V2989SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | SPM600 |
| Purity | Protein G affinity chromatography |
| UniProt | P42081 |
| Localization | Cytoplasmic, membrane |
| Applications | Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT |
| Limitations | This CD86 antibody is available for research use only. |
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CD86 is a receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. [UniProt]
Optimal dilution of the CD86 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Recombinant human full-length protein was used as the immunogen for the CD86 antibody.
Store the CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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