CD86 Antibody FITC Conjugate | V2056FITC NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2056FITC-100T	500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 Tests	539
Formulation

Flow cytometry testing of PFA-fixed human Ramos cells with unlabeled CD86 antibody (clone BU63); Red=isotype control, Blue= CD86 antibody.

Species Reactivity	Human
Format	FITC Conjugate
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	BU63
Purity	Protein G affinity chromatography
Gene ID	942 (Human)
Localization	Cell surface
Applications	Flow Cytometry : 5ul/test/million cells Immunofluorescence : 1:50-1:100
Limitations	This CD86 antibody FITC conjugate is available for research use only.
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Description

This antibody recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.