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Home >> Antibodies >> CD86 Antibody

CD86 Antibody [clone C86/3713] (V8523)

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  Catalog No Formulation Size Price (USD)  
Image V8523-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V8523-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V8523SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human tonsil with CD86 antibody (clone C86/3713). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with CD86 antibody (clone C86/3713). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Ramos cell lysate with CD86 antibody (clone C86/3713). Expected molecular weight: 38-70 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/3713) as confirmation of integrity and purity.
Western blot testing of human Raji cell lysate with CD86 antibody (clone C86/3713). Expected molecular weight: 38-70 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name C86/3713
Purity Protein G affinity chromatography
UniProt P4208
Localization Cytoplasmic, membrane
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD86 antibody is available for research use only.
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Description

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.

Application Notes

Optimal dilution of the CD86 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 66-195 from the human protein was used as the immunogen for the CD86 antibody.

Storage

Store the CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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