• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> CD86 Antibody

CD86 Antibody [clone C86/3711] (V8759)

  Catalog No Formulation Size Price (USD)  
Image V8759-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
Image
V8759-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
Image
V8759SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
Bulk quote request
IHC staining of FFPE human lung carcinoma with CD86 antibody (clone C86/3711). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma with CD86 antibody (clone C86/3711). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Ramos cell lysate with CD86 antibody (clone C86/3711). Expected molecular weight: 38-70 kDa depending on glycosylation level.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CD86 antibody (clone C86/3711). These results demonstrate the foremost specificity of the C86/3711 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name C86/3711
Purity Protein G affinity chromatography
UniProt P4208
Localization Cytoplasmic, membrane
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CD86 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : FACS, IF, IHC-P
    Reactivity : Human, Mouse, Rat
    Recmousemono
  • Applications : ELISA, FACS, IF, WB, IHC-P
    Reactivity : Human, Mouse, Rat
    Recrabbitmono
  • Applications : IHC-P, WB, IF/ICC, FACS
    Reactivity : Human. Other species not tested.
    Recrabbitmono
  • Applications : IHC-P
    Reactivity : Human
    Recrabbitmono
  • Applications : IHC-P
    Reactivity : Human
    Recmousemono
  • Applications : WB, IHC-P
    Reactivity : Human
    Microvalidated
  • Applications : FACS
    Reactivity : Human
    Citation  Citations(10)
  • Applications : Functional studies, FACS, IF, IHC-P
    Reactivity : Human, Mouse, Rat
    Citation  Citations(11)
  • Applications : FACS, IF
    Reactivity : Human, Mouse and Rat. Other species not known.
    Citation  Citations(11)
  • Applications : IF, WB, IHC-P
    Reactivity : Human
  • Applications : IF, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB, ELISA
    Reactivity : Mouse, Rat

Description

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.

Application Notes

Optimal dilution of the CD86 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 66-195 from the human protein was used as the immunogen for the CD86 antibody.

Storage

Store the CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.